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Welcome to world of manga.Explore and read manga, manhwa, and doujin manga (doujin is Japanese for doujinshi) is short for “original webtoon” or “online comic book.” Manga are the Japanese equivalent of comics and traditionally are drawn and written as comic art. When it comes to online manga, these works are the same as conventional manga but are legally prohibited in many countries. Manhwa refers to webtoons which are Korean equivalent of manhwa. Read more about what is manhwa.Construction and evaluation of a Tn7 end point transposon library.
The transposon Tn7 is widely used in bacteria, especially Mycobacterium tuberculosis. Here, we describe the construction of a library of mobilizable Tn7 end point in M. tuberculosis H37Rv. By random insertion of Tn7 to occur within a spectrum of mutations in genes, Tn7 in the library bears a special characteristic, transposition frequency (TF). In addition, we also developed a high-efficiency liposome transfection system for M. tuberculosis to make the mutant library. The mutant library that we have constructed is very suitable for mutational analysis and also other genetic applications.Q:

Can you show a particular “there is no continuous function” proof?

If there is no continuous function that takes values in $\mathbb{Q}\cap [0,1]$ and is strictly increasing on $\mathbb{Q}$ this can be proven by taking any two irrationals $x,y$ and any rational $q$. It is easy to show that $f$ is continuous at $q$ because the distance between the graphs of $f$ and the continuous function $f(x)=1+\frac{x-q}{x-q+1}$ will be $0$ (uniform distance between the two functions). However, is it possible to show that it is continuous at $x$?

A:

If the function is continuous in rational numbers, i.e., $f(p)=f(q)$ for all rational $p$ and $q$, then $f$ must be continuous in real numbers.
Suppose not. Then there exists $r,s\in\mathbb{R}$ with \$r

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