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UMS 5.0 BG Version By IZoSa Final

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. and 64-bit.Role of natural killer cells in the down-regulation of murine cytomegalovirus immediate-early transcription.
Recent studies suggest that natural killer (NK) cells are capable of down-regulating early gene expression of cytomegalovirus (CMV) and that the timing of response appears to be important for the expression of certain early viral genes. We examined this hypothesis in vivo using a murine model of CMV infection in which it is possible to follow the kinetics of the antiviral response of NK cells and the regulation of early CMV gene expression. In this model, infection of BALB/c mice with murine cytomegalovirus (MCMV) stimulates a rapid NK cell-mediated antiviral response. Inoculation of mice with recombinant MCMV, which harbors a green fluorescent protein (GFP)-fusion reporter gene controlled by the CMV immediate-early (IE) gene promoter, permitted in vivo NK cell killing of GFP-MCMV-infected cells to be followed. Infected, but not uninfected, NK cells were inactivated and dead NK cells were cleared rapidly, with efficient GFP expression from the CMV IE promoter. Three days following virus inoculation, control IE GFP(+) cells in the liver and spleen were detectable by immunohistochemistry. In contrast, G

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